CTNNA1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257408
Artikelname: CTNNA1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1257408
Hersteller Artikelnummer: orb1257408
Alternativnummer: BYT-ORB1257408-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human CTNNA1 (NP_001894.2).
Konjugation: Unconjugated
Alternative Synonym: Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, CTNNA1
CTNNA1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 115kDa
UniProt: P35221
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: CTNNA1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using CTNNA1 antibody (orb1257408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using CTNNA1 antibody (orb1257408) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using CTNNA1 antibody (orb1257408) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CTNNA1 antibody (orb1257408) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CTNNA1 antibody (orb1257408) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 3 ug CTNNA1 antibody (orb1257408). Western blot was performed from the immunoprecipitate using CTNNA1 antibody (orb1257408) at a dilution of 1:500.