PGRMC1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257416
Artikelname: PGRMC1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1257416
Hersteller Artikelnummer: orb1257416
Alternativnummer: BYT-ORB1257416-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 44-195 of human PGRMC1 (NP_006658.1).
Konjugation: Unconjugated
Alternative Synonym: PGRMC1, HPR6.6, MPR
PGRMC1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 24kDa
UniProt: O00264
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: PGRMC1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using PGRMC1 antibody (orb1257416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using PGRMC1 antibody (orb1257416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using PGRMC1 antibody (orb1257416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using PGRMC1 antibody (orb1257416) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PGRMC1 antibody (orb1257416) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using PGRMC1 Polyclonal Antibody (orb1257416) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using PGRMC1 Polyclonal Antibody (orb1257416) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using PGRMC1 Polyclonal Antibody (orb1257416) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.