FUBP1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257425
Artikelname: FUBP1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1257425
Hersteller Artikelnummer: orb1257425
Alternativnummer: BYT-ORB1257425-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 302-644 of human FUBP1 (NP_003893.2).
Konjugation: Unconjugated
Alternative Synonym: FUBP1, FBP, FUBP
FUBP1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 79kDa
UniProt: Q96AE4
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: FUBP1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using FUBP1 antibody (orb1257425) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using FUBP1 antibody (orb1257425) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using FUBP1 antibody (orb1257425) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using FUBP1 antibody (orb1257425) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using FUBP1 antibody (orb1257425) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug FUBP1 antibody (orb1257425). Western blot was performed from the immunoprecipitate using FUBP1 antibody (orb1257425) at a dilution of 1:1000.