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NONO Antibody, Unconjugated, Rabbit, Polyclonal

NONO Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257581

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Artikelname:	NONO Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1257581
Hersteller Artikelnummer:	orb1257581
Alternativnummer:	BYT-ORB1257581-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NONO (NP_001138881.1).
Konjugation:	Unconjugated
Alternative Synonym:	NONO, P54, NMT55, NRB54, P54NRB

NONO Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 58kDa
UniProt:	Q15233
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	NONO

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using Nono antibody (orb1257581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat liver using Nono Antibody (orb1257581) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using Nono Antibody (orb1257581) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using Nono Antibody (orb1257581) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using Nono antibody (orb1257581).

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