NHEJ1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257619
Artikelname: NHEJ1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1257619
Hersteller Artikelnummer: orb1257619
Alternativnummer: BYT-ORB1257619-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1).
Konjugation: Unconjugated
Alternative Synonym: NHEJ1, nonhomologous end-joining factor 1, FLJ12610, XLF, Cernunnos, XRCC4-like factor
NHEJ1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 38kDa
UniProt: Q9H9Q4
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: NHEJ1
Anwendungsbeschreibung: Application Notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using NHEJ1 antibody (orb1257619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human breast cancer using NHEJ1 antibody (orb1257619) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using NHEJ1 antibody (orb1257619) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using NHEJ1 antibody (orb1257619) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2OS cells using NHEJ1 antibody (orb1257619) at dilution of 1:100. Blue: DAPI for nuclear staining.