Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
Konjugation:
Unconjugated
Alternative Synonym:
CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3, insulin-like growth factor 2 mRNA-binding protein 3, IGF-II mRNA-binding protein 3, IGF2 mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, VICKZ family member 3, cancer/testis antigen 98
Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (orb1257695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human esophagus using IGF2BP3 antibody (orb1257695) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using IGF2BP3 antibody (orb1257695) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (orb1257695) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100 ug extracts of HepG2 cells using 3 ug IGF2BP3 antibody (orb1257695). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (orb1257695) at a dilution of 1:1000.
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