NDUFB10 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257782
Artikelname: NDUFB10 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1257782
Hersteller Artikelnummer: orb1257782
Alternativnummer: BYT-ORB1257782-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human NDUFB10 (NP_004539.1).
Konjugation: Unconjugated
Alternative Synonym: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, Complex I-PDSW, CI-PDSW, NADH-ubiquinone oxidoreductase PDSW subunit, NDUFB10
NDUFB10 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 21kDa
UniProt: O96000
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: NDUFB10
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using NDUFB10 antibody (orb1257782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse stomach using NDUFB10 antibody (orb1257782) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NDUFB10 antibody (orb1257782) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NDUFB10 antibody (orb1257782) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug NDUFB10 antibody (orb1257782). Western blot was performed from the immunoprecipitate using NDUFB10 antibody (orb1257782) at a dilution of 1:1000.