OCLN Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
BYT-ORB1258098
- Bilder (6)
| Artikelname: | OCLN Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | BYT-ORB1258098 |
| Hersteller Artikelnummer: | orb1258098 |
| Alternativnummer: | BYT-ORB1258098-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human Occludin (NP_002529.1). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Occludin, OCLN |
| OCLN Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | batch dependent |
| Molekulargewicht: | Observed: 59kDa |
| UniProt: | Q16625 |
| Puffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Formulierung: | Liquid |
| Target-Kategorie: | OCLN |
| Anwendungsbeschreibung: | Application Notes: WB: 1:1000 - 1:2000 |
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Western blot analysis of extracts of various cell lines, using Occludin antibody (orb1258098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
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Immunohistochemistry of paraffin-embedded rat pancreas using Occludin antibody (orb1258098) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse kidney using Occludin antibody (orb1258098) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using Occludin antibody (orb1258098) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using Occludin antibody (orb1258098) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of PC12 cells using Occludin antibody (orb1258098) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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