SIAH1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1258154
Artikelname: SIAH1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1258154
Hersteller Artikelnummer: orb1258154
Alternativnummer: BYT-ORB1258154-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SIAH1 (NP_001006611.1).
Konjugation: Unconjugated
Alternative Synonym: SIAH1, SIAH1A
SIAH1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 31kDa
UniProt: Q8IUQ4
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: SIAH1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of mouse kidney, using SIAH1 antibody (orb1258154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using SIAH1 antibody (orb1258154) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SIAH1 antibody (orb1258154) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using SIAH1 antibody (orb1258154) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SIAH1 antibody (orb1258154). Blue: DAPI for nuclear staining.