Western blot analysis of extracts of mouse kidney, using SIAH1 antibody (orb1258154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using SIAH1 antibody (orb1258154) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SIAH1 antibody (orb1258154) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using SIAH1 antibody (orb1258154) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SIAH1 antibody (orb1258154). Blue: DAPI for nuclear staining.
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