H4K20me3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
BYT-ORB1258176
- Bilder (6)
| Artikelname: | H4K20me3 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | BYT-ORB1258176 |
| Hersteller Artikelnummer: | orb1258176 |
| Alternativnummer: | BYT-ORB1258176-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ChIP, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse, Other, Rat |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4 |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA |
| H4K20me3 Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | batch dependent |
| Molekulargewicht: | Observed: 11kDa |
| UniProt: | P62805 |
| Puffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Formulierung: | Liquid |
| Target-Kategorie: | H4K20me3 |
| Anwendungsbeschreibung: | Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H4-K20 antibody (orb1258176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
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Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody (orb1258176). |
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Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H4-K20 antibody (orb1258176) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H4-K20 antibody (orb1258176) at dilution of 1:200 (40x lens). |
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Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 antibody (orb1258176). Blue: DAPI for nuclear staining. |
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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H4-K20 antibody (orb1258176) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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