SMYD1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1258199
Artikelname: SMYD1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1258199
Hersteller Artikelnummer: orb1258199
Alternativnummer: BYT-ORB1258199-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human SMYD1 (NP_938015.1).
Konjugation: Unconjugated
Alternative Synonym: Histone-lysine N-methyltransferase SMYD1, SET and MYND domain-containing protein 1, SMYD1
SMYD1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 57kDa
UniProt: Q8NB12
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: SMYD1
Anwendungsbeschreibung: Application Notes: WB: 1:200 - 1:2000IHC: 1:20 - 1:200IF: 1:20 - 1:100
Western blot analysis of extracts of various cell lines, using SMYD1 Antibody (orb1258199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using SMYD1 antibody (orb1258199) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using SMYD1 antibody (orb1258199) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using SMYD1 antibody (orb1258199) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using SMYD1 antibody (orb1258199). Blue: DAPI for nuclear staining.