EEF1A1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1258518
Artikelname: EEF1A1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1258518
Hersteller Artikelnummer: orb1258518
Alternativnummer: BYT-ORB1258518-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 123-462 of human EEF1A1 (NP_001393.1).
Konjugation: Unconjugated
Alternative Synonym: EEF1A1, CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1, EE1A1
EEF1A1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
UniProt: P68104
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: EEF1A1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using EEF1A1 antibody (orb1258518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using EEF1A1 antibody (orb1258518) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using EEF1A1 antibody (orb1258518) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using EEF1A1 antibody (orb1258518) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using EEF1A1 antibody (orb1258518) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EEF1A1 antibody (orb1258518) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using EEF1A1 antibody (orb1258518) at dilution of 1:100. Blue: DAPI for nuclear staining.