Western blot analysis of extracts of various cell lines, using MLKL antibody (orb1258731) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using MLKL antibody (orb1258731) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MLKL antibody (orb1258731) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using MLKL antibody (orb1258731) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using MLKL antibody (orb1258731) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using MLKL antibody (orb1258731) at dilution of 1:200 (40x lens).
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