CCNB1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
BYT-ORB1259192
- Bilder (7)
| Artikelname: | CCNB1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | BYT-ORB1259192 |
| Hersteller Artikelnummer: | orb1259192 |
| Alternativnummer: | BYT-ORB1259192-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172.1). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CCNB1, CCNB |
| CCNB1 Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | batch dependent |
| Molekulargewicht: | Observed: 55kDa |
| UniProt: | P14635 |
| Puffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Formulierung: | Liquid |
| Target-Kategorie: | CCNB1 |
| Anwendungsbeschreibung: | Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
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Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (orb1259192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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Immunohistochemistry of paraffin-embedded rat testis using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human breast cancer using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse testis using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of 293T cells using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug Cyclin B1 antibody (orb1259192). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (orb1259192) at a dilution of 1:1000. |
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