PRPF19 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1260836
Artikelname: PRPF19 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1260836
Hersteller Artikelnummer: orb1260836
Alternativnummer: BYT-ORB1260836-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRPF19 (NP_055317.1).
Konjugation: Unconjugated
Alternative Synonym: PRPF19, NMP200, PRP19, PSO4, UBOX4, hPSO4, SNEV
PRPF19 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 55kDa
UniProt: Q9UMS4
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: PRPF19
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using PRPF19 antibody (orb1260836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug PRPF19 antibody (orb1260836). Western blot was performed from the immunoprecipitate using PRPF19 antibody (orb1260836) at a dilution of 1:1000.