Osteocalcin Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB1266
- Bilder (7)
| Artikelname: | Osteocalcin Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB1266 |
| Hersteller Artikelnummer: | orb1266 |
| Alternativnummer: | BYT-ORB1266-50,BYT-ORB1266-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Osteocalcin (21-100/100aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | BGP, OC, OCN, Bglap2, Bgpr, Bgpra, OSTCN_BOVIN, BGLAP, Bone Gla protein (BGP), Gamma-carboxyglutamic acid-containing protein, OSTCN_HUMAN, OSTCN_PIG, OSTCN_RAT, |
| Osteocalcin Rabbit Polyclonal Antibody |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
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citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:400 overnight at 4C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium clei. |
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Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining. |
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Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei stainin. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human MG63 cell lysates, Lane 3: Human A549 cell lysates, Lane 4: Human U-2 OS cell lysates, Primary: Anti-Osteocalcin (orb1266) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 11 kDa. |
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