This EphA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 875-904 amino acids from the C-terminal region of human EphA4.
Application Notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
Western blot analysis of lysates from Hela, HUVEC cell line (from left to right), using EPHA4 Antibody at 1:1000 at each lane.
Western blot analysis of lysates from Hela, NCI-H460, HUVEC cell line (from left to right), using EPHA4 Antibody at 1:1000 at each lane.
Western blot analysis of lysates from Hela, NCI-H460, mouse NIH/3T3 cell line and human ovary tissue lysate (from left to right), using EPHA4 Antibody at 1:1000 at each lane.
Western blot analysis of anti-EphA4 Pab in NCI-H460 cell lysate
Western blot analysis of EPHA4 using EphA4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma, HC = hepatocarcinoma.
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