To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with antigen affinity purified anti-human Eotaxin-2 (orb1272559) as the detection antibody at a concentration of approximately 1.0-2.0 ug/ml.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary.
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