Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (orb1274434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 antibody (orb1274434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 Antibody (orb1274434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 Antibody (orb1274434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (orb1274434) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EEF1B2 antibody (orb1274434). Blue: DAPI for nuclear staining.
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