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HMGB1 Antibody, Unconjugated, Rabbit, Polyclonal

HMGB1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1274463

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Artikelname:	HMGB1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1274463
Hersteller Artikelnummer:	orb1274463
Alternativnummer:	BYT-ORB1274463-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).
Konjugation:	Unconjugated
Alternative Synonym:	HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1

HMGB1 Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 29kDa
UniProt:	P09429
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	HMGB1

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using HMGB1 antibody (orb1274463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody (orb1274463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (orb1274463) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (orb1274463) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (orb1274463) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using HMGB1 antibody (orb1274463) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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