Synthetic peptide / encompassing a sequence within the C-terminus region.
Konjugation:
Unconjugated
Rabbit polyclonal antibody to c-MET
Klonalität:
Polyclonal
Puffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target-Kategorie:
MET / C-MET / HGFR
Application Verdünnung:
Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:200, Immunohistochemistry (Paraffin) 1:100
All lanes: Anti c-MET antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4C.
All lanes: Anti c-MET antibody at 1/700 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4C.
All lanes: Anti c-MET antibody at 1/700 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4C.
All lanes: Anti c-MET antibody at 1/800 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4C.
All lanes: Anti c-MET antibody at 1/800 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4C.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling c-MET with orb1294310 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling c-MET with orb1294310 at 1/100.
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