Synthetic peptide / encompassing a sequence within the N-terminus region.
Konjugation:
Unconjugated
Rabbit polyclonal antibody to p63
Klonalität:
Polyclonal
Puffer:
100mM Tris Glycine, 1%rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target-Kategorie:
p63
Application Verdünnung:
Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:300, Immunohistochemistry (Paraffin) 1:100
All lanes: Anti-p63 antibody - at 1/500 dilution, Lysates/proteins at 50 µg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA an hour before being incubated with orb1294319 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294319 anti-p63 antibody (red) at 1:100 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling p63 with orb1294319 at 1/100.
Immunohistochemical analysis of paraffin embedded Human tissue labeling p63 with orb1294319 at 1/100.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling p63 with orb1294319 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling p63 with orb1294319 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling p63 with orb1294319 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
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