Angiotensin II type 1A receptor Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB13241
Artikelname: Angiotensin II type 1A receptor Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB13241
Hersteller Artikelnummer: orb13241
Alternativnummer: BYT-ORB13241-50,BYT-ORB13241-100,BYT-ORB13241-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Angiotensin II type 1A receptor (101-200/359aa)
Konjugation: Unconjugated
Alternative Synonym: AG2S, AGTR1B, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, ATR1, HAT1R, 1810074K20Rik, AT1a, AT2R1A, Agt1ar, Agtr-1a, Agtr1, Angtr-1a, AT2R1B, Agtr-1b, Angtr-1b, AT3, AT1R, AGTR1_HUMAN, Angiotensin II type-1 receptor (AT1 receptor), AGTR1A, AGTRA_MOUSE, Angiotensin II type-1 receptor A (AT1 receptor A), AGTRB_MOUSE, Angiotensin II type-1 receptor B (AT1 receptor B), AGTRA_RAT, AGTRB_RAT,
Angiotensin II type 1A receptor Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 41 kDa
UniProt: P30556
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: AGTR1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of human lung carcinoma tissue using Angiotensin II antibody.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (orb13241) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (orb13241) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: A549 (Human) Cell Lysate at 430 ug, Primary: Anti-Angiotensin II type 1A receptor (orb13241) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41/51 kD.
Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Placenta (Mouse) Lysate at 40 ug, Lane 4: Kidney (Mouse) Lysate at 40 ug, Lane 5: Embryo (Mouse) Lysate at 40 ug, Lane 6: Cerebrum (Mouse) Lysate at 40 ug, Lane 7: NIH/3T3 (Mouse) cell Lysate at 30 ug, Lane 8: Liver (Rat) Lysate at 40 ug, Lane 9: Heart (Rat) Lysate at 40 ug, Lane 10: Kidney (Rat) Lysate at 40 ug, Lane 11: Cerebrum (Rat) Lysate at 40 ug, Lane 12: Embryo (Rat) Lysate at 40 ug, Primary: Anti-Angiotensin II type 1A receptor (orb13241) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 46 kD.
Sample: Liver (Mouse) Lysate at 40 ug, Lung (Mouse) Lysate at 40 ug, Adrenal gland (Rat) Lysate at 40 ug, Primary: Anti-Angiotensin II type 1A receptor (orb13241) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 56 kD.