HDAC3 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB13470
- Bilder (7)
| Artikelname: | HDAC3 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB13470 |
| Hersteller Artikelnummer: | orb13470 |
| Alternativnummer: | BYT-ORB13470-50,BYT-ORB13470-100,BYT-ORB13470-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human HDAC3 (351-428/428aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | HD3, KDAC3, RPD3, RPD3-2, HDAC3_HUMAN, HDAC3, Protein deacetylase HDAC3, Protein deacylase HDAC3, SMAP45, 3.5.1.98, HDAC3_MOUSE, HDAC3_RAT, |
| HDAC3 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 47 kDa |
| UniProt: | O15379 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | HDAC3 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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issue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-HDAC3/HD3 Polyclonal Antibody, Unconjugated (orb13470) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HDAC3) Polyclonal Antibody, Unconjugated (orb13470) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-HDAC3/HD3 (orb13470) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 47 kDa. |
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Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-HDAC3 (orb13470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD. |
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Sample: Uterus (Mouse) Lysate at 40 ug, Ovary (Mouse) Lysate at 40 ug, Primary: Anti-HDAC3 (orb13470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 50 kD. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-HDAC3/HD3 Polyclonal Antibody, Unconjugated (orb13470) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-HDAC3/HD3 Polyclonal Antibody, Unconjugated (orb13470) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (red) used at 1:200 dilution for 40 minutes at 37C. |
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