MASP2 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB13562
Artikelname: MASP2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB13562
Hersteller Artikelnummer: orb13562
Alternativnummer: BYT-ORB13562-50,BYT-ORB13562-100,BYT-ORB13562-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human MASP2 (351-450/686aa)
Konjugation: Unconjugated
Alternative Synonym: MAP-2, MAP19, MASP-2, MASP1P1, sMAP, MASP2_HUMAN, MASP2, MBL-associated serine protease 2, Mannose-binding protein-associated serine protease 2 (MASP-2), 3.4.21.104,
MASP2 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 74 kDa
UniProt: O00187
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: MASP2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat liver using MASP2 antibody.
Immunohistochemical staining of rat ileum tissue using MASP2 antibody.
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MASP2) Polyclonal Antibody, Unconjugated (orb13562) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MASP2) Polyclonal Antibody, Unconjugated (orb13562) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lung (Mouse) Lysate at 40 ug, Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-MASP2 (orb13562) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 74 kD.
Sample: Plasma (Rat) Lysate at 40 ug, Primary: Anti-MASP2 (orb13562) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 75 kD.



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