0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:
Liquid
Target-Kategorie:
FLT4
Application Verdünnung:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Blank control: A549 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).
Cell: (mo) Splenocyte (2% BSA at 4blocked for 30 minutes. 1% Paraformaldehyde fixed for 30 minutes, 0.1% TritonX-100 permeablized for 2 minutes). Concentration: 1:100. Incubation: 40 minutes. Host/Blank: Splenocytes. Flow cytometric analysis of Rabbit Anti-VEGFR3 antibody (orb13753) (green) compared with control in the absence of primary antibody (blue) followed by Splenocytes. Secondary antibody: Goat Anti-rabbit IgG/FITC antibody (orb868805).
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VEGFR3) Polyclonal Antibody, Unconjugated (orb13753) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-VEGFR3 Polyclonal Antibody, Unconjugated (orb13753) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Flow cytometric analysis of A549 cells using VEGFR3 antibody.
Flow cytometric analysis of Splenocyte cell using VEG
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