ZNF307/ZKSCAN3 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB13769
- Bilder (7)
| Artikelname: | ZNF307/ZKSCAN3 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB13769 |
| Hersteller Artikelnummer: | orb13769 |
| Alternativnummer: | BYT-ORB13769-50,BYT-ORB13769-100,BYT-ORB13769-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human ZNF307 (351-450/545aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | P1P373C6, ZNF307, ZNF427, ZSCAN36, ZKSC4_HUMAN, ZKSCAN4, P373c6.1, Zinc finger protein 307, Zinc finger protein 427, |
| ZNF307/ZKSCAN3 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 62 kDa |
| UniProt: | Q969J2 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | ZKSCAN4 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
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Blank control (black line): SH-SY5Y. Primary Antibody (green line): Rabbit Anti-ZNF307/ZKSCAN3 antibody (orb13769), Dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Sample: K562 (Human) Cell Lysate at 40 ug, Primary: Anti-ZNF307/ZKSCAN3 (orb13769) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD. |
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SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ZNF307/ZKSCAN3) polyclonal Antibody, Unconjugated (orb13769) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated (orb13769) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse fetal liver, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated (orb13769) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated (orb13769) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated (orb13769) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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