FTO Recombinant Rabbit Monoclonal Antibody, Clone: [19H6], Unconjugated

Artikelnummer: BYT-ORB1499264
Artikelname: FTO Recombinant Rabbit Monoclonal Antibody, Clone: [19H6], Unconjugated
Artikelnummer: BYT-ORB1499264
Hersteller Artikelnummer: orb1499264
Alternativnummer: BYT-ORB1499264-100,BYT-ORB1499264-25,BYT-ORB1499264-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: A synthesized peptide derived from human FTO 45687/505
Konjugation: Unconjugated
Alternative Synonym: FTO_HUMAN, FTO, Fat mass and obesity-associated protein, U6 small nuclear RNA (2-O-methyladenosine-N(6)-)-demethylase FTO, U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, mRNA (2-O-methyladenosine-N(6)-)-demethylase FTO (m6A(m)-demethylase FTO), mRNA N(6)-methyladenosine demethylase FTO, tRNA N1-methyl adenine demethylase FTO, KIAA1752, FTO_MOUSE, Protein fatso,
FTO Recombinant Rabbit Monoclonal Antibody
Klonalität: Recombinant
Konzentration: 1mg/ml
Klon-Bezeichnung: [19H6]
Molekulargewicht: 58 kDa
UniProt: Q9C0B1
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: FTO
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (orb1499264) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (orb1499264) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (orb1499264) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human U87MG cell lysates, Lane 4: Human 293T cell lysates, Lane 5: Human SW480 cell lysates, Primary: Anti-FTO (orb1499264) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kDa, Observed band size: 62 kDa.
IHC-P analysis of human glioma using FTO Monoclonal Antibody at 1:200 dilution.



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