SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody, Clone: [R3F5], Unconjugated
Artikelnummer:
BYT-ORB1499273
- Bilder (8)
| Artikelname: | SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody, Clone: [R3F5], Unconjugated |
| Artikelnummer: | BYT-ORB1499273 |
| Hersteller Artikelnummer: | orb1499273 |
| Alternativnummer: | BYT-ORB1499273-100,BYT-ORB1499273-25,BYT-ORB1499273-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human Sequestosome 1 (410-440/440aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | A170, DMRV, EBIAP, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B, OSF-6, Osi, STAP, STONE14, ZIP, SQSTM_HUMAN, SQSTM1, EBI3-associated protein of 60 kDa (EBIAP | p60), Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62 (p62), ORCA, SQSTM_MOUSE, Ubiquitin-binding protein p62, SQSTM_RAT, Protein kinase C-zeta-interacting protein (PKC-zeta-interacting protein), |
| SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [R3F5] |
| Molekulargewicht: | 47 kDa |
| UniProt: | Q13501 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | SQSTM1 |
| Application Verdünnung: | WB=1:2000-20000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:100-500, IF=1:200-1000, Flow-Cyt=1ug/Test |
|
|
25 ug total protein per lane of various lysates probed with SQSTM1/p62 monoclonal antibody, unconjugated (orb1499273) at 1:5000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. |
|
|
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (SQSTM1/p62) monoclonal Antibody, Unconjugated (orb1499273) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
|
|
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SQSTM1/p62) Monoclonal Antibody, Unconjugated (orb1499273) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SQSTM1/p62) Monoclonal Antibody, Unconjugated (orb1499273) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SQSTM1/p62) Monoclonal Antibody, Unconjugated (orb1499273) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (mouse spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SQSTM1/p62) Monoclonal Antibody, Unconjugated (orb1499273) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SQSTM1/p62) Monoclonal Antibody, Unconjugated (orb1499273) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. Primary Antibody (green): Rabbit Anti-SQSTM1/p62 antibody (orb1499273): 1 µg/10 6 cells, Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (orb868805): 1 µg/Test. Isotype Control (orange): Rabbit IgG (orb570250). Blank control (black): PBS. Acquisition of 20000 events was performed. |
Produktgarantie und fachkundiger Support
