N Cadherin Recombinant Rabbit Monoclonal Antibody, Clone: [5G1], Unconjugated
Artikelnummer:
BYT-ORB1499395
- Bilder (6)
| Artikelname: | N Cadherin Recombinant Rabbit Monoclonal Antibody, Clone: [5G1], Unconjugated |
| Artikelnummer: | BYT-ORB1499395 |
| Hersteller Artikelnummer: | orb1499395 |
| Alternativnummer: | BYT-ORB1499395-100,BYT-ORB1499395-25,BYT-ORB1499395-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human Cadherin 2 (160-200aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ACOGS, ADHD8, ARVD14, CD325, CDHN, CDw325, NCAD, N-CAD, N-cadherin, CADH2_HUMAN, CDH2, Neural cadherin (N-cadherin), CADH2_MOUSE, CADH2_RAT, |
| N Cadherin Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [5G1] |
| Molekulargewicht: | 100 kDa |
| UniProt: | P19022 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | CDH2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: HeLa, Mouse brain, Rat brain, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 100 kDa, Observed MW: 125 kDa. |
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Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Human PANC-1 cell lysates, Lane 5: Human A549 cell lysates, Primary: Anti-N Cadherin (orb1499395) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kDa, Observed band size: 140 kDa. |
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Tissue: Human endometrium, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1499395. |
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Tissue: Human liver, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1499395. |
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Tissue: Mouse liver, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1499395. |
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Tissue: Rat liver, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1499395. |
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