IGF2BP1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB1518
Artikelname: IGF2BP1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB1518
Hersteller Artikelnummer: orb1518
Alternativnummer: BYT-ORB1518-50,BYT-ORB1518-100,BYT-ORB1518-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human IGF2BP1/IMP1 (31-130/577aa)
Konjugation: Unconjugated
Alternative Synonym: ZBP1, CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, D030026A21Rik, D11Moh40e, D11Moh45, Neilsen, ZBP-1, mir-3063, fb96d05, wu:fb18f03, wu:fb96d05, wu:fi72a06, zgc:152963, IF2B1_CHICK, IGF2BP1, IGF2 mRNA-binding protein 1, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zip-code binding polypeptide, Zipcode-binding protein 1 (ZBP-1), IF2B1_HUMAN, Coding region determinant-binding protein (CRD-BP), IF2B1_MOUSE, IF2B1_RAT, B-actin zipcode-binding protein 1 (ZBP1 | rZBP-1), IF2B1_DANRE,
IGF2BP1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 63 kDa
UniProt: Q9NZI8
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: IGF2BP1
Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of mouse embryo tissue using IGF2BP1 antibody.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IGF2BP1) Polyclonal Antibody, Unconjugated (orb1518) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (IGF2BP1) Polyclonal Antibody, Unconjugated (orb1518) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IGF2BP1) Polyclonal Antibody, Unconjugated (orb1518) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IGF2BP1) Polyclonal Antibody, Unconjugated (orb1518) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-IGF2BP1 Polyclonal Antibody, Unconjugated (orb1518) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.