Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Reinheit:
The monoclonal antibody was purified from cell culture supernatant
Formulierung:
Liquid
Target-Kategorie:
Granzyme B
Application Verdünnung:
Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10 6 cells. Immunocytochemistry (ICC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 -
Anwendungsbeschreibung:
Application Notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
Detection of human Granzyme B by western blot. Samples: Whole cell lysate (15 µg) from HEK293T, HeLa, SR, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GranzymeB recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody
Detection of human Granzyme B by immunohistochemistry. Sample: FFPE section of lymph node. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG
Detection of human Granzyme B by immunocytochemistry. Sample: FFPE section of Granzyme B over-expressing cells (left) and Granzyme H over-expressing cells (right). Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG
Detection of human Granzyme B (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: Opal(TM). Counterstain: DAPI (blue)
Detection of human Granzyme B (shaded) in SR cells by flow cytometry. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG
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