Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Liquid
Target-Kategorie:
Ki-67
Application Verdünnung:
Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10 6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. ICC-IF 1:100 - 1:500. Immunohisto
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human Ki-67 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-Ki-67 recombinant monoclonal (orb1519949) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human Ki-67 by immunocytochemistry. Sample: FFPE section of MOLT-4 cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human Ki-67 by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:100. Secondary: DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.
Detection of human Ki-67 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human Ki-67 (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal(TM). Counterstain: DAPI (blue).
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