Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Liquid
Target-Kategorie:
CD3E
Application Verdünnung:
WB 1:1000, IP: 20 µl/mg lysate, IHC: 1:100 - 1:500, Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC: Fixed in 4
Anwendungsbeschreibung:
Application Notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
Detection of human CD3E by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, K-562, SK-MEL-28, MOLT-4, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH
Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB
Detection of human CD3E by immunhistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody used at 1:250. Secondary: DyLight 594-conjugated goat anti-rabbit IgG
Detection of human CD3E (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: Opal(TM). Counterstain: DAPI (blue)
Detection of human CD3E by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-CD3E recombinant monoclonal antibody (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG
Detection of human CD3E in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD3E Recombinant Monoclonal Antibody (orb1519964) Secondary: Goat anti-Rabbit IgG Heavy and Light Chain Highly Cross-Adsorbed Antibody (HRP) (orb1531012). Substrate: DAB.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
