Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Liquid
Target-Kategorie:
CD20
Application Verdünnung:
FC: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10 6 cells. Immunohistochemistry (IHC): 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF: 1:100 - 1:500. Epitope r
Anwendungsbeschreibung:
Application Notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
Detection of human CD3E (yellow) , C
Detection of human CD20 by western blot. Samples: Whole cell lysate (25 µg) from Ramos and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD20 monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody
Detection of human CD20 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Purified mouse monoclonal anti-CD20 antibody used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue)
Detection of human CD20 (shaded) in Raji cells by flow cytometry. Antibody: Mouse anti-CD20 monoclonal antibody or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG
Detection of human CD20 by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD20 antibody used at 1:250. Secondary: DyLight 594-conjugated goat anti-mouse IgG
Detection of human CD20 by immunocytochemistry. Sample: FFPE section of VLN3G2 cells. Antibody: Purified mouse monoclonal anti-CD20 antibody used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue)
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