Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Liquid
Target-Kategorie:
KAP-1 Phospho (S824)
Application Verdünnung:
WB - 1:2,000 - 1:10,000, IP - 2 - 10 µg/mg lysate, IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., ICC - 1:200 - 1:1,000, ICC-IF - 1:200 - 1:1,000
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human Phospho KAP-1 (S824) by ICC.Samples: HeLa cells with MOCK CIP treatment (left) and with CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) at 1:200.
Detection of human Phospho KAP-1 (S824) by ICC.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200. Detection: Red fluorescent anti-rabbit IgG-DyLight 594 conjugated.
Detection of human Phospho KAP-1 (S824) by IHC.Samples: FFPE serial sections of prostate carcinoma with MOCK CIP treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) at a dilution of 1:200.
Detection of human phospho KAP-1 (S824) by western blot of immunoprecipitates.Samples: HEK293T cells. Antibodies: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody and rabbit anti-KAP-1 recombinant monoclonal antibody.
Detection of human phospho KAP-1 (S824) by western blot.Samples: Whole cell lysate from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody at 0.04 µg/ml.
Detection of mouse phospho KAP-1 (S824) by western blot.Samples: Whole cell lysate from NIH 3T3 cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody at 0.04 µg/ml.
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