5HT7 Receptor Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB155538
Artikelname: 5HT7 Receptor Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB155538
Hersteller Artikelnummer: orb155538
Alternativnummer: BYT-ORB155538-50,BYT-ORB155538-100,BYT-ORB155538-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human 5HT7 Receptor/SR-7 (51-150/479aa)
Konjugation: Unconjugated
Alternative Synonym: 5-HT7, 5-HT-X, 5Ht7, 5HT7R_CAVPO, HTR7, 5-HT-7, Serotonin receptor 7, 5HT7R_HUMAN, 5HT7R_MOUSE, 5HT7R_RAT, GPRFO,
5HT7 Receptor Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 54 kDa
Pubmed: 30639027
UniProt: P34969
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: HTR7
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (orb155538) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (orb155538) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (orb155538) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Hippocampus (Mouse) Lysate at 40 ug, Hippocampus (Rat) Lysate at 40 ug, Primary: Anti-5HT7 Receptor (orb155538) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Cerebellum tissue lysates, Primary: Anti-5HT7 Receptor (orb155538) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 50 kDa.
Western blot analysis of Mouse & Rat Hippocampus Lysate using 5HT7 Receptor antibody.



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