Arginase II Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB155720
- Bilder (8)
| Artikelname: | Arginase II Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB155720 |
| Hersteller Artikelnummer: | orb155720 |
| Alternativnummer: | BYT-ORB155720-50,BYT-ORB155720-100,BYT-ORB155720-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human Arginase II (181-290/354aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | AII, ARGI2_HUMAN, ARG2, Arginase II, Kidney-type arginase, Non-hepatic arginase, Type II arginase, 3.5.3.1, ARGI2_MOUSE, ARGI2_RAT, |
| Arginase II Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 36 kDa |
| UniProt: | P78540 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | ARG2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
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Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-Arginase II antibody (orb155720), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Arginase II) polyclonal Antibody, Unconjugated (orb155720) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Arginase II) Polyclonal Antibody, Unconjugated (orb155720) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Arginase II) Polyclonal Antibody, Unconjugated (orb155720) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: DU145 (human) cell Lysate at 30 ug, Primary: Anti-Arginase II (orb155720) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36kD, Observed band size: 36 kD. |
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Sample: Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-Arginase II (orb155720) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD. |
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Sample: Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-Arginase II at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD. |
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Tissue/Cell: human MCF-7 cells, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Arginase II Polyclonal Antibody, Unconjugated (orb155720) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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