Phospho-Ataxin 1 (Ser775) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB155744
- Bilder (6)
| Artikelname: | Phospho-Ataxin 1 (Ser775) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB155744 |
| Hersteller Artikelnummer: | orb155744 |
| Alternativnummer: | BYT-ORB155744-50,BYT-ORB155744-100,BYT-ORB155744-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human Ataxin 1 around the phosphorylation site of Ser776 RW(p-S)AP |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ATXN1 (p-S775), p-ATXN1, phospho-ATXN1, ATX1, D6S504E, SCA1, 2900016G23Rik, Gm10786, ATX1_HUMAN, ATXN1, Spinocerebellar ataxia type 1 protein, ATX1_MOUSE, Spinocerebellar ataxia type 1 protein homolog, |
| Phospho-Ataxin 1 (Ser775) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 87 kDa |
| UniProt: | P54253 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | ATXN1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Ataxin 1 (Ser775)) Polyclonal Antibody, Unconjugated (orb155744) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Ataxin 1 (Ser775)) Polyclonal Antibody, Unconjugated (orb155744) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Ataxin 1 (Ser775)) Polyclonal Antibody, Unconjugated (orb155744) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Ataxin 1 (Ser775)) Polyclonal Antibody, Unconjugated (orb155744) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Ataxin 1 (Ser775)) Polyclonal Antibody, Unconjugated (orb155744) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Ataxin 1 (Ser775) (orb155744) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 87 kD, Observed band size: 87 kD. |
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