BBS1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB155794
Artikelname: BBS1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB155794
Hersteller Artikelnummer: orb155794
Alternativnummer: BYT-ORB155794-50,BYT-ORB155794-100,BYT-ORB155794-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human BBS5 (181-270/593aa)
Konjugation: Unconjugated
Alternative Synonym: BBS2L2, D19Ertd609e, BBS1_HUMAN, BBS1, BBS2-like protein 2, Bardet-Biedl syndrome 1 protein,
BBS1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 65 kDa
UniProt: Q8NFJ9
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: BBS1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (BBS1) Polyclonal Antibody, Unconjugated (orb155794) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (BBS1) Polyclonal Antibody, Unconjugated (orb155794) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-BBS1 (orb155794) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.
Sample: HepG2 (Human) Cell Lysate at 30 ug, U2os (Human) Cell Lysate at 30 ug, Primary: Anti-BBS1 (orb155794) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.
U87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (BBS1) polyclonal Antibody, Unconjugated (orb155794) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
IHC-P analysis of rat brain using BBS1 Polyclonal Antibody at 1:200 dilution.



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