CD53 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB156312

Artikelname:	CD53 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:	BYT-ORB156312
Hersteller Artikelnummer:	orb156312
Alternativnummer:	BYT-ORB156312-50,BYT-ORB156312-100,BYT-ORB156312-200
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	KLH conjugated synthetic peptide derived from human CD53 (65-160/219aa)
Konjugation:	Unconjugated
Alternative Synonym:	MOX44, TSPAN25, CD53_HUMAN, CD53, Cell surface glycoprotein CD53, Tetraspanin-25 (Tspan-25),

CD53 Rabbit Polyclonal Antibody

Klonalität:	Polyclonal
Konzentration:	1mg/ml
Molekulargewicht:	24 kDa
UniProt:	P19397
Puffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:	Liquid
Target-Kategorie:	CD53

Application Verdünnung:

WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

	Immunohistochemical staining of human lung carcinoma tissue using CD53 antibody.
	Immunohistochemical staining of human colon cancer tissue using CD53 antibody.
	Sample: Jurkat (Human) Cell Lysate at 30 ug, Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-CD53 (orb156312) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 24 kD.
	Sample: MOLT-4 (Human) Cell Lysate at 30 ug, Primary: Anti-CD53 (orb156312) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 25 kD.
	Sample: Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-CD53 (orb156312) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD.
	Tissue/Cell: human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CD53 Polyclonal Antibody, Unconjugated (orb156312) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Menge 50 ul 241,00 €
100 ul 375,00 €
200 ul 452,00 €

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