CLN8 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB156404
Artikelname: CLN8 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB156404
Hersteller Artikelnummer: orb156404
Alternativnummer: BYT-ORB156404-50,BYT-ORB156404-100,BYT-ORB156404-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human CLN8 (201-286/286aa)
Konjugation: Unconjugated
Alternative Synonym: C8orf61, EPMR, TLCD6, mnd, CLN8_CANLF, CLN8, CLN8_HUMAN, CLN8_MOUSE,
CLN8 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 33 kDa
UniProt: Q9UBY8
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: CLN8
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat brain tissue using CLN8 antibody.
Immunohistochemical staining of Rat uterus tissue using CLN8 antibody.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CLN8) Polyclonal Antibody, Unconjugated (orb156404) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CLN8) Polyclonal Antibody, Unconjugated (orb156404) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-CLN8 (orb156404) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.
Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CLN8 Polyclonal Antibody, Unconjugated (orb156404) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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