CYB5R3 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB156504
Artikelname: CYB5R3 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB156504
Hersteller Artikelnummer: orb156504
Alternativnummer: BYT-ORB156504-50,BYT-ORB156504-100,BYT-ORB156504-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human CYB5R3 (101-200/301aa)
Konjugation: Unconjugated
Alternative Synonym: B5R, DIA1, Nadhcb5, RNNADHCB5, NB5R3_HUMAN, CYB5R3, Cytochrome b5 reductase, Diaphorase-1, 1.6.2.2, NB5R3_RAT,
CYB5R3 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 34 kDa
UniProt: P00387
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: CYB5R3
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CYB5R3) Polyclonal Antibody, Unconjugated (orb156504) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human myocardium), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CYB5R3) Polyclonal Antibody, Unconjugated (orb156504) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Blood (Mouse) Cell Lysate at 30 ug, Primary: Anti-CYB5R3 (orb156504) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.
Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Rat Liver tissue lysates, Lane 3: Rat Placenta tissue lysates, Primary: Anti-CYB5R3 (orb156504) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kDa, Observed band size: 32 kDa.
Sample: Spinal cord (Mouse) Lysate at 40 ug, Primary: Anti-CYB5R3 (orb156504) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.
IHC-P analysis of human liver using CYB5R3 Polyclonal Antibody at 1:200 dilution.
Western blot:Spinal cord (Mouse),Primary:Anti-CYB5R3 at 1:1000 dilution.



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