ERCC8 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB156770
Artikelname: ERCC8 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB156770
Hersteller Artikelnummer: orb156770
Alternativnummer: BYT-ORB156770-50,BYT-ORB156770-100,BYT-ORB156770-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human ERCC8/CSA (161-260/396aa)
Konjugation: Unconjugated
Alternative Synonym: CKN1, CSA, UVSS2, 2410022P04Rik, 2810431L23Rik, 4631412O06Rik, B130065P18Rik, ERCC8_HUMAN, ERCC8, Cockayne syndrome WD repeat protein CSA, ERCC8_MOUSE, Cockayne syndrome WD repeat protein CSA homolog,
ERCC8 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 44 kDa
UniProt: Q13216
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: ERCC8
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Western blot analysis of mouse testis lysate using ERCC8 antibody.
Western blot analysis of extracts from Testis using ERCC8
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERCC8) Polyclonal Antibody, Unconjugated (orb156770) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERCC8) Polyclonal Antibody, Unconjugated (orb156770) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERCC8) Polyclonal Antibody, Unconjugated (orb156770) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-ERCC8 (orb156770) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 44 kD.
Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-ERCC8 (orb156770) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 442 kD.



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