EXOSC10 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB156799
Artikelname: EXOSC10 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB156799
Hersteller Artikelnummer: orb156799
Alternativnummer: BYT-ORB156799-50,BYT-ORB156799-100,BYT-ORB156799-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from human EXOSC10/PMSCL2 (41-140/885aa)
Konjugation: Unconjugated
Alternative Synonym: PM-Scl, PM/Scl-100, PMSCL, PMSCL2, RRP6, Rrp6p, p2, p3, p4, EXOSX_HUMAN, EXOSC10, Autoantigen PM/Scl 2, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, Polymyositis/scleroderma autoantigen 100 kDa (PM/Scl-100), Polymyositis/scleroderma autoantigen 2, 3.1.13.-, EXOSX_MOUSE, Autoantigen PM/Scl 2 homolog, Polymyositis/scleroderma autoantigen 2 homolog,
EXOSC10 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 101 kDa
UniProt: Q01780
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: EXOSC10
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of human skin tissue using EXOSC10 antibody.
Immunohistochemical staining of mouse lymph tissue using EXOSC10 antibody.
Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EXOSC10) Polyclonal Antibody, Unconjugated (orb156799) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-EXOSC10 (orb156799) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 101 kD, Observed band size: 103 kD.
Tissue/Cell: human skin tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-EXOSC10, Unconjugated (orb156799) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Mouse Lymph nodes, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-EXOSC10, Unconjugated (orb156799) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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