Phospho-FHOD1 (Thr1141) Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB156869
Artikelname: Phospho-FHOD1 (Thr1141) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB156869
Hersteller Artikelnummer: orb156869
Alternativnummer: BYT-ORB156869-50,BYT-ORB156869-100,BYT-ORB156869-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Rat
Immunogen: KLH conjugated synthesised phosphopeptide derived from human FHOD1 around the phosphorylation site of Thr1141 RR(p-T)LK
Konjugation: Unconjugated
Alternative Synonym: FHOD1 (p-T1141), p-FHOD1, phospho-FHOD1, FHOS, FHOD1_HUMAN, FHOD1, Formin homolog overexpressed in spleen 1 (FHOS), Formin homology 2 domain-containing protein 1, FHOS1,
Phospho-FHOD1 (Thr1141) Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 126 kDa
UniProt: Q9Y613
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: FHOD1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Anwendungsbeschreibung: Modification: Phosphorylated
Immunohistochemical staining of rat heart tissue using FHOD1 (Phospho-Thr1141) antibody.
Immunohistochemical staining of rat heart tissue using FHOD1 (Phospho-Thr1141) antibody.
Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-FHOD1 (Thr1141) (orb156869) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 126 kD, Observed band size: 126 kD.
Sample: K562 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-FHOD1 (Thr1141) (orb156869) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 126 kD, Observed band size: 126 kD.
Tissue/Cell: rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-FHOD1 Polyclonal Antibody, Unconjugated (orb156869) 1:600, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-FHOD1 Polyclonal Antibody, Unconjugated (orb156869) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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