GPR91 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB157370
Artikelname: GPR91 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB157370
Hersteller Artikelnummer: orb157370
Alternativnummer: BYT-ORB157370-50,BYT-ORB157370-100,BYT-ORB157370-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from human GPR91 (131-334/334aa)
Konjugation: Unconjugated
Alternative Synonym: GPR91, SUCR1_HUMAN, SUCNR1, G-protein coupled receptor 91, P2Y purinoceptor 1-like, SUCR1_MOUSE, SUCR1_RAT,
GPR91 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 39 kDa
UniProt: Q9BXA5
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: SUCNR1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of human kidney tissue using GPR91 antibody.
Western blot analysis of extracts from Liver tissue(mo) using GPR91 antibody.
Protein: 1.kidney lyates (mo), 2.liver lyates (mo), Primary: Rabbit Anti-GPR91 (orb157370) at 1:300, Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG (H+L) at 1: 10000, Predicted band size: 39 kD, Observed band size: 49 kD.
Sample: HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-GPR91 (orb157370) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39kD, Observed band size: 39kD.
Tissue/Cell: human breast cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated (orb157370) 1:600, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated (orb157370) 1:600, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Western blot analysis of extracts from Liver tissue (mo) using GPR91 Antibody.



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