Neurabin 2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB158484
- Bilder (6)
| Artikelname: | Neurabin 2 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB158484 |
| Hersteller Artikelnummer: | orb158484 |
| Alternativnummer: | BYT-ORB158484-50,BYT-ORB158484-100,BYT-ORB158484-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Spinophilin/Neurabin 2 (358-460/815aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PPP1R6, PPP1R9, SPINO, Spn, SPL, Neb2, NEB2_HUMAN, PPP1R9B, Neurabin-II, Protein phosphatase 1 regulatory subunit 9B, Spinophilin, NEB2_MOUSE, NEB2_RAT, Neural tissue-specific F-actin-binding protein II, PP1bp134, p130, |
| Neurabin 2 Rabbit Polyclonal Antibody |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Neurabin 2) Polyclonal Antibody, Unconjugated (orb158484) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Neurabin 2) Polyclonal Antibody, Unconjugated (orb158484) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Neurabin 2) Polyclonal Antibody, Unconjugated (orb158484) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Neurabin 2) Polyclonal Antibody, Unconjugated (orb158484) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Cerebellum tissue lysates, Primary: Anti-Neurabin 2 (orb158484) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 89 kDa, Observed band size: 115 kDa. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Neurabin 2 Polyclonal Antibody, Unconjugated (orb158484) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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