This product is Rabbit IgG / APC, also known as Rabbit Immunoglobulin G/APC Isotype Control.
Puffer:
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% glycerol.
Formulierung:
Liquid
Blank control: HUVEC. Isotype control: Rabbit IgG-APC (orb1610934), dilution: 1 ug/Test, Primary Antibody (green line): Rabbit Anti-CD34 antibody, dilution: 1 ug/Test, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Isotype control antibody (orange line) was rabbit IgG. Acquisition of 20000 events was performed.
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