This product is Mouse IgG / FITC, also known as Mouse Immunoglobulin G/FITC.
Puffer:
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% glycerol.
Formulierung:
Liquid
Blank control: Hela. Isotype control: Mouse IgG-FITC (orb1610935), dilution: 1 ug/Test, Primary Antibody (green line): Mouse Anti-EGFR antibody, dilution: 1 ug/Test, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Isotype control antibody (orange line) was mouse IgG. Acquisition of 20000 events was performed.
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